pFGA1 vector (V006271)

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LOCUS       40924_19966       10639 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fungal expression vector pFGA1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10639)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10639)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10639)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10639)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    420..5015
                     /label=5' flanking region of A. niger glaA
                     /note="5' flanking region of A. niger glaA"
     regulatory      5016..6124
                     /label=A. niger glaA promoter
                     /note="A. niger glaA promoter"
                     /regulatory_class="promoter"
     misc_feature    6125..6165
                     /label=5' UTR of A. niger glaA
                     /note="5' UTR of A. niger glaA"
     exon            6166..6379
                     /note="exon 1 of A. niger glaA"
     intron          6380..6454
                     /note="intron 1 of A. niger glaA"
     exon            6455..6741
                     /note="exon 2 of A. niger glaA"
     intron          6742..6796
                     /note="intron 2 of A. niger glaA"
     exon            6797..6893
                     /note="exon 3 of A. niger glaA"
     intron          6894..6954
                     /note="intron 3 of A. niger glaA"
     exon            6955..7592
                     /note="exon 4 of A. niger glaA"
     intron          7593..7650
                     /note="intron 4 of A. niger glaA"
     exon            7651..8337
                     /note="exon 5 of A. niger glaA"
     misc_feature    8338..8393
                     /label=3' UTR of A. niger glaA
                     /note="3' UTR of A. niger glaA"
     primer_bind     complement(8418..8434)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8442..8458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8466..8496)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8511..8532)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8820..9408)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9582..10439)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10440..10544)
                     /label=AmpR promoter