pFF838 vector (V006272)

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LOCUS       V006272                 4004 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006272
VERSION     V006272
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Farzadfard F, Lu TK.
  TITLE     Synthetic biology. Genomically encoded analog memory with precise in
            vivo DNA writing in living cell populations
  JOURNAL   Science 346 (6211), 1256272 (2014)
   PUBMED   25395541
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Farzadfard F, Lu T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2014) Biological Engineering, MIT, 77
            Massachusetts Avenue, NE47, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 4004)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4004)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science";
            date: "2014"; volume: "346"; issue: "6211"; pages: "1256272"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-DEC-2014) Biological Engineering, MIT, 77 Massachusetts Avenue,
            NE47, Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..80
                     /label="PLtetO-1 promoter"
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     misc_feature    89..170
                     /note="primer for the Ec86 RT; msr"
     misc_feature    complement(160..293)
                     /note="template for Ec86 RT; msd(lacZ_ON)"
     CDS             313..1272
                     /gene="ret"
                     /label="Retron Ec86 reverse transcriptase"
                     /note="Retron Ec86 reverse transcriptase from Escherichia
                     coli. Accession#: P23070"
     CDS             1312..2094
                     /label="Beta"
                     /note="single-stranded DNA binding recombinase in the
                     lambda Red system"
     terminator      2115..2201
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2370..2915)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(3029..3123)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3147..3803)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3804..3906)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"