pFF714 vector (V006280)

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LOCUS       V006280                 4024 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006280
VERSION     V006280
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Farzadfard F, Lu TK.
  TITLE     Synthetic biology. Genomically encoded analog memory with precise in
            vivo DNA writing in living cell populations
  JOURNAL   Science 346 (6211), 1256272 (2014)
   PUBMED   25395541
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Farzadfard F, Lu T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2014) Biological Engineering, MIT, 77
            Massachusetts Avenue, NE47, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 4024)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4024)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science";
            date: "2014"; volume: "346"; issue: "6211"; pages: "1256272"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-DEC-2014) Biological Engineering, MIT, 77 Massachusetts Avenue,
            NE47, Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..33
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    40..56
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    97..178
                     /note="primer for the Ec86 RT; msr"
     misc_feature    complement(168..301)
                     /note="template for Ec86 RT; msd(galK_OFF)"
     CDS             321..1280
                     /gene="ret"
                     /label="Retron Ec86 reverse transcriptase"
                     /note="Retron Ec86 reverse transcriptase from Escherichia
                     coli. Accession#: P23070"
     CDS             1320..2102
                     /label="Beta"
                     /note="single-stranded DNA binding recombinase in the
                     lambda Red system"
     terminator      2123..2209
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2373..2961)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(3049..3143)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3167..3823)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3824..3926)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"