pFE604T vector (V006284)

LOCUS       40924_19901       10504 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Conjugative cloning vector pFE604T DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Yong HT, Yamamoto N, Takeuchi R, Hsieh YJ, Conrad TM, Datsenko KA, 
            Nakayashiki T, Wanner BL, Mori H.
  TITLE     Development of a System for Discovery of Genetic Interactions for 
            Essential Genes in Escherichia coli K-12
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10504)
  AUTHORS   Mori H, Yong HT.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2013) Contact:Hirotada Mori Nara Institute of 
            Science and Technology, Graduate School of Biological Sciences; 
            8916-5 Takayama, Ikoma, Nara 630-0101, Japan URL 
            :http://ecoli.naist.jp/Lab
REFERENCE   3  (bases 1 to 10504)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10504)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-AUG-2013) Contact:Hirotada Mori Nara Institute of Science and 
            Technology, Graduate School of Biological Sciences; 8916-5 Takayama,
            Ikoma, Nara 630-0101, Japan URL :http://ecoli.naist.jp/Lab"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..67
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    44..60
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    complement(74..93)
                     /label=lac operator (symmetric)
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     RBS             110..121
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      502..596
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        933..961
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             1131..1661
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     oriT            1684..2018
     rep_origin      complement(2037..2425)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(2489..3112)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     promoter        3128..3183
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     CDS             3191..4393
                     /label=TcR
                     /note="tetracycline efflux protein"
     misc_feature    complement(4851..5324)
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(5400..6368)
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             complement(6371..7543)
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    complement(7869..8119)
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(8125..8877)
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     rep_origin      complement(8968..9187)
                     /direction=LEFT
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     promoter        9331..9408
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             9409..10488
                     /label=lacI
                     /note="lac repressor"