pFD1146 vector (V006286)

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LOCUS       40924_19891        9400 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Bacteroides fragilis shuttle vector pFD1146, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Parker AC, Jeffrey Smith C.
  TITLE     Development of an IPTG inducible expression vector adapted for 
            Bacteroides fragilis
  JOURNAL   Plasmid 68 (2), 86-92 (2012)
  PUBMED    22487080
REFERENCE   2  (bases 1 to 9400)
  AUTHORS   Smith CJ.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 9400)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9400)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2012"; volume: "68"; issue: "2"; pages: "86-92"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-MAR-2012) Dept. Microbiology "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(786..1466)
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="replication protein for replication in Bacteroides"
                     /protein_id="AFI41181.1"
                     /translation="MILNRKIIFPLILFLWKIIVYFVENNISQKHIKMENKKAVKLTDF
                     QKKQENPFMKTSYRGIENHVVKKYKSNSGGDKRAVVALADTETGEVFKTSFIRQIEVDE
                     EQFTKLYLSNFAAFFDLSQAAIRVFGYFMTCMKPKNDLIIFNRKKCLEYTKYKTDKAVY
                     KGLAELVKAEIIARGPADNLWFINPLIVFNGDRVTFAKTYVSEKDFSCPKERRSRETTI
                     ITWL"
     gene            complement(786..1466)
                     /gene="repA"
                     /label=repA
     CDS             2552..3352
                     /codon_start=1
                     /gene="ermF"
                     /product="ErmF"
                     /label=ermF
                     /note="erythromycin resistance protein"
                     /protein_id="AFI41182.1"
                     /translation="MTKKKLPVRFTGQHFTIDKVLIKDAIRQANISNQDTVLDIGAGKG
                     FLTVHLLKIANNVVAIENDTALVEHLRKLFSDARNVQVVGCDFRNFAVPKFPFKVVSNI
                     PYGITSDIFKILMFESLGNFLGGSIVLQLEPTQKLFSRKLYNPYTVFYHTFFDLKLVYE
                     VGPESFLPPPTVKSALLNIKRKHLFFDFKFKAKYLAFISYLLEKPDLSVKTALKSIFRK
                     SQVRSISEKFGLNLNAQIVCLSPSQWLNCFLEMLEVVPEKFHPS"
     gene            2552..3352
                     /gene="ermF"
                     /label=ermF
     CDS             4208..4975
                     /codon_start=1
                     /gene="aad9"
                     /product="Aad9"
                     /label=aad9
                     /note="spectinomycin resistance protein"
                     /protein_id="AFI41183.1"
                     /translation="MRRIYLNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNS
                     DLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPPKQ
                     EFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFSDVR
                     RAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERI
                     LLAVRSYLGENIEWTNENVNLTINYLNNRLKKL"
     gene            4208..4975
                     /gene="aad9"
                     /label=aad9
     rep_origin      5818..6406
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            6727..6836
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             6869..7237
                     /label=traJ
                     /note="oriT-recognizing protein"
     protein_bind    7472..7493
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7508..7538
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7546..7562
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7570..7586
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7611..7631
                     /label=LacI, LacO3 binding site
                     /bound_moiety="LacI, LacO3"
     regulatory      7678..7699
                     /gene="cfxA"
                     /regulatory_class="promoter"
     gene            7678..7699
                     /gene="cfxA"
                     /label=cfxA
     protein_bind    7712..7732
                     /label=LacI, LacO1 binding site
                     /bound_moiety="LacI, LacO1"
     protein_bind    7714..7730
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             8292..9371
                     /label=lacI
                     /note="lac repressor"