pFB-Neo-LacZ vector (V006293)

Download GeneBank File(.gb)

LOCUS       40924_19771        9609 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFB-Neo-LacZ, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Jaskula J, Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2001) Technical Services, Stratagene, 11011 N 
            Torrey Pines Rd, La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 9609)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9609)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (29-JUN-2001) Technical Services, Stratagene, 11011 N Torrey Pines 
            Rd, La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             289..758
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    823..1180
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1245..1661
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1671..2044
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     misc_feature    5167..5629
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             5634..5663
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             5768..5785
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             5806..6579
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     LTR             6642..7235
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    7446..7586
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7772..8360)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8534..9391)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9392..9496)
                     /label=AmpR promoter