pFB-Neo-E-hA20 vector (V006294)

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LOCUS       V006294                 9033 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006294
VERSION     V006294
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9033)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9033)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9033)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9033)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      169..760
                     /label="5' MMLV-LTR"
                     /note="5' MMLV-LTR"
                     /regulatory_class="promoter"
     LTR             289..758
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    823..1180
                     /label="MMLV Psi"
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1245..1661
                     /label="gag (truncated)"
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1671..2045
                     /label="pol region"
                     /note="Moloney murine leukemia virus (MMLV) pol region
                     containing the splice acceptor site"
     misc_feature    2077..2115
                     /label="E-tag"
                     /note="E-tag"
     CDS             2125..4494
                     /gene="TNFAIP3"
                     /label="Tumor necrosis factor alpha-induced protein 3"
                     /note="Tumor necrosis factor alpha-induced protein 3 from
                     Homo sapiens. Accession#: P21580"
     misc_feature    4498..4541
                     /label="3' UTR of hA20"
                     /note="3' UTR of hA20"
     misc_feature    4591..5053
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             5058..5087
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             5192..5209
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     CDS             5225..6016
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     LTR             6079..6672
                     /label="LTR"
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    6883..7023
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7209..7797)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7971..8828)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8829..8933)
                     /label="AmpR promoter"