pFAGN173 vector (V006302)

LOCUS       40924_19681        4980 bp DNA     circular SYN 18-DEC-2018
DEFINITION  BiFC vector pFAGN173, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Gong Z, Lu H.
  TITLE     A new combination of fluorescent protein fragments suitable for 
            green-colored bimolecular fluorescence complementation (BiFC) assays
            in Schizosaccharomyces pombe
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Gong Z, Lu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2012) School of Life Science, Fudan University, 
            Room 618, Science Building, Handan Road 220, Shanghai 200433, China
REFERENCE   3  (bases 1 to 4980)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4980)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-DEC-2012) School of Life Science, Fudan University, Room 618, 
            Science Building, Handan Road 220, Shanghai 200433, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        235..253
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    303..317
                     /label=linker
                     /note="linker"
     CDS             318..830
                     /label=VN173
                     /note="N-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
     CDS             831..857
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     regulatory      867..1359
                     /label=Saccharomyces cerevisiae LEU2 terminator
                     /note="Saccharomyces cerevisiae LEU2 terminator"
                     /regulatory_class="terminator"
     gene            1405..2761
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     promoter        complement(2869..2887)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3145..3733)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3907..4764)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4765..4869)
                     /label=AmpR promoter