pFA545 vector (V006373)

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LOCUS       40924_19161       10079 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mariner transposase delivery vector pFA545, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10079)
  AUTHORS   Bae T, Banger AK, Wallace A, Glass EM, Aslund F, Schneewind O, 
            Missiakas DM.
  TITLE     Staphylococcus aureus virulence genes identified by bursa aurealis 
            mutagenesis and nematode killing
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12312-12317 (2004)
  PUBMED    15304642
REFERENCE   2  (bases 1 to 10079)
  AUTHORS   Bae T, Aslund F, Missiakas DM, Schneewind O.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2004) Molecular Genetics and Cell Biology, 
            Committee on Microbiology, University of Chicago, 920 E. 58th St., 
            Chicago, IL 60637, USA
REFERENCE   3  (bases 1 to 10079)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10079)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2004"; volume: "101"; issue: "33"; pages:
            "12312-12317"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUL-2004) Molecular Genetics and Cell Biology, Committee on 
            Microbiology, University of Chicago, 920 E. 58th St., Chicago, IL 
            60637, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1703..1719)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1727..1743
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1751..1781)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1796..1817)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2105..2693)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2867..3724)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3725..3829)
                     /label=AmpR promoter
     promoter        4619..4637
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             4995..6146
                     /codon_start=1
                     /gene="xylR"
                     /product="Xyl repressor"
                     /label=xylR
                     /protein_id="AAU04865.1"
                     /translation="MENNFIVNENEKRVLKQIFNNSNISRTQISKNLELNKATISNILN
                     NLKHKSLVNEVGEGNSTKSGGRKPILLEINQKYGYYISMDLTYDSVELMYNYFDATILK
                     QDSYELNDKNVSSILQILKSNINVSEKYDTLYGLLGISISIHGIVDDEQNIINLPFHKN
                     EKRTFTDELKSFTNVPVVIENEANLSALYEKSLYINSNINNLITLSIHKGIGAGIIINK
                     KLYRGSNGEAGEIGKTLVLESINNNDNKYYKIEDICSQDALIQKINNRLGVTLTFTELI
                     QYYNEGNSIVAHEIKQFINKMTVLIHNLNTQFNPDAIYINCPLINELPNILNEIKEQFS
                     CFSQGSPIQLHLTTNVKQATLLGGTLAIMQKTLNINNIQMNIK"
     gene            4995..6146
                     /gene="xylR"
                     /label=xylR
     gene            6197..7249
                     /gene="tnp"
                     /label=tnp
     CDS             6197..7243
                     /codon_start=1
                     /gene="tnp"
                     /product="mariner transposase"
                     /label=tnp
                     /protein_id="AAU04866.1"
                     /translation="MEKKEFRVLIKYCFLKGKNTVEAKTWLDNEFPDSAPGKSTIIDWY
                     AKFKRGEMSTEDGERSGRPKEVVTDENIKKIHKMILNDRKMKLIEIAEALKISKERVGH
                     IIHQYLDMRKLCAKWVPRELTFDQKQQRVDDSERCLQLLTRNTPEFFRRYVTMDETWLH
                     HYTPESNRQSAEWTATGEPSPKRGKTQKSAGKVMASVFWDAHGIIFIDYLEKGKTINSD
                     YYMALLERLKVEIAAKRPHMKKKKVLFHQDNAPCHKSLRTMAKIHELGFELLPHPPYSP
                     DLAPSDFFLFSDLKRMLAGKKFGCNEEVIAETEAYFEAKPKEYYQNGIKKLEGRYNRCI
                     ALEGNYVE"
     CDS             complement(7822..8280)
                     /codon_start=1
                     /gene="tetD"
                     /product="polypeptide D"
                     /label=tetD
                     /note="tetracycline resistance protein"
                     /protein_id="AAU04867.1"
                     /translation="MMKTIYHVNVATIGNSVIFPGTMSVIVFGYFGGFLVDRKGSLFVF
                     ILGSLSISISFLTIAFFVEFSMWLTTFMFIFVMGELSFTKTVISKIVSSSLSEEEVASG
                     MSLLNFTSFLSEGTGIAIVGGLLSLQLINRKLVLEFINYSSGVYSNIL"
     gene            complement(7822..8280)
                     /gene="tetD"
                     /label=tetD
     CDS             complement(8237..9124)
                     /codon_start=1
                     /gene="tetB"
                     /product="polypeptide B"
                     /label=tetB
                     /note="tetracycline resistance protein"
                     /protein_id="AAU04868.1"
                     /translation="MFSLYKKFKGLFYSVLFWLCILSFFSVLNEMVLNVSLPDIANHFN
                     TTPGITNWVNTAYMLTFSIGTAVYGKLSDYINIKKLLIIGISLSCLGSLIAFIGHNHFF
                     ILIFGRLVQGVGSAAFPSLIMVVVARNITRKKQGKAFGFIGSIVALGEGLGPSIGGIIA
                     HYIHWSYLLILPMITIVTIPFLIKVMVPGKSTKNTLDIVGIVLMSISIICFMLFTTNYN
                     WTFLILFTIFFVIFIKHISRVSNPFINPKLGKNIPFMLGLFSGWANIFYSSWFYINGAL
                     YDENYLSCKCSDNR"
     gene            complement(8237..9124)
                     /gene="tetB"
                     /label=tetB