pFA-LIFEACT-GFP-URA3 vector (V006382)

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LOCUS       V006382                 5074 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006382
VERSION     V006382
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M.
  TITLE     Actin dynamics and endocytosis in the absence of Arp2/3 in Candida
            albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2011) Biology, McGill University, 1205 Docteur
            Penfield, Montreal, Quebec H3A 1B1, Canada
REFERENCE   3  (bases 1 to 5074)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5074)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-MAY-2011) Biology, McGill University, 1205 Docteur Penfield,
            Montreal, Quebec H3A 1B1, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             151..201
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et
                     al., 2008)"
                     /label="Lifeact"
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             226..936
                     /label="GFP"
                     /note="green fluorescent protein"
     regulatory      940..1195
                     /label="Saccharomyces cerevisiae URA3 terminator"
                     /note="Saccharomyces cerevisiae URA3 terminator"
                     /regulatory_class="terminator"
     CDS             1598..2407
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     promoter        complement(2712..2730)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2988..3576)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3750..4607)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4608..4712)
                     /label="AmpR promoter"
     promoter        5058..5074
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"