Basic Vector Information
- Vector Name:
- pFA-HAH1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6162 bp
- Type:
- Functional Analysis vector
- Replication origin:
- ori
- Source/Author:
- Paul S, Ganesan K.
pFA-HAH1 vector Map
pFA-HAH1 vector Sequence
LOCUS 40924_19106 6162 bp DNA circular SYN 18-DEC-2018 DEFINITION Functional Analysis vector pFA-HAH1, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6162) AUTHORS Paul S, Ganesan K. TITLE Single-Transformation Gene Function Test by Conditional Expression of Both Alleles of Candida albicans Genes JOURNAL Unpublished REFERENCE 2 (bases 1 to 6162) AUTHORS Paul S, Ganesan K. TITLE Direct Submission JOURNAL Submitted (01-NOV-2010) Institute of Microbial Technology, Sector 39-A, Chandigarh, UT 160036, India REFERENCE 3 (bases 1 to 6162) TITLE Direct Submission REFERENCE 4 (bases 1 to 6162) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (01-NOV-2010) Institute of Microbial Technology, Sector 39-A, Chandigarh, UT 160036, India" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..6162 /mol_type="other DNA" /organism="synthetic DNA construct" gene 47..915 /gene="HIS1" /label=HIS1 repeat_region 562..915 gene 921..2871 /gene="ARG4" /label=ARG4 /note="derived from Candida albicans ARG4" CDS 1378..2784 /codon_start=1 /gene="ARG4" /product="argininosuccinate lyase" /EC_number="4.3.2.1" /label=ARG4 /protein_id="AEQ75399.1" /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL TGTKVYTQGLNKLGLITTEELNLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDALGGTAKSAVLK QLENLKSILS" gene 2876..3729 /gene="HIS1" /label=HIS1 repeat_region 2876..3229 promoter complement(3800..3818) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" rep_origin complement(4076..4664) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4838..5695) /label=AmpR /note="beta-lactamase" promoter complement(5696..5800) /label=AmpR promoter promoter 6146..6162 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase"
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