pFA-HAH1-MET3prom vector (V006385)

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LOCUS       40924_19101        7531 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Functional Analysis vector pFA-HAH1-MET3prom, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Paul S, Ganesan K.
  TITLE     Single-Transformation Gene Function Test by Conditional Expression 
            of Both Alleles of Candida albicans Genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7531)
  AUTHORS   Paul S, Ganesan K.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2010) Institute of Microbial Technology, Sector 
            39-A, Chandigarh, UT 160036, India
REFERENCE   3  (bases 1 to 7531)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7531)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-NOV-2010) Institute of Microbial Technology, Sector 39-A, 
            Chandigarh, UT 160036, India"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            47..915
                     /gene="HIS1"
                     /label=HIS1
     repeat_region   562..915
     gene            921..2871
                     /gene="ARG4"
                     /label=ARG4
                     /note="derived from Candida albicans ARG4"
     CDS             1378..2784
                     /codon_start=1
                     /gene="ARG4"
                     /product="argininosuccinate lyase"
                     /EC_number="4.3.2.1"
                     /label=ARG4
                     /protein_id="AEQ75403.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELNLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDALGGTAKSAVLK
                     QLENLKSILS"
     gene            2876..3729
                     /gene="HIS1"
                     /label=HIS1
     repeat_region   2876..3229
     regulatory      3734..5132
                     /label=MET3 promoter from Candida albicans
                     /note="MET3 promoter from Candida albicans"
                     /regulatory_class="promoter"
     promoter        complement(5169..5187)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(5445..6033)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6207..7064)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7065..7169)
                     /label=AmpR promoter
     promoter        7515..7531
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"