pF12K RM Flexi vector (V006393)

LOCUS       40924_19021        3465 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pF12K RM Flexi, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Vidugiriene J, Schenborn E, McNamara B.
  TITLE     Regulated Mammalian Expression System Technical Manual, TM289
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Vidugiriene J, Schenborn E, McNamara B.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2006) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 3465)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3465)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-SEP-2006) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Road, Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..309
                     /label=12X lambda operator
                     /note="12X lambda operator"
     regulatory      322..434
                     /label=minimal CMV promoter
                     /note="minimal CMV promoter"
                     /regulatory_class="promoter"
     CDS             477..809
                     /label=barnase
                     /note="ribonuclease from Bacillus amyloliquefaciens"
     misc_feature    822..877
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     polyA_signal    complement(982..1103)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             1502..2293
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     rep_origin      complement(2468..3056)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3172..3455
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"