Basic Vector Information
- Vector Name:
- pEXPR49
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7758 bp
- Type:
- Mammalian expression vector
- Replication origin:
- ori
- Source/Author:
- Siciliano V.
- Promoter:
- EF-1α
pEXPR49 vector Vector Map
pEXPR49 vector Sequence
LOCUS V006414 7758 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V006414
VERSION V006414
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 7758)
AUTHORS Siciliano V.
TITLE Engineering modular intracellular protein sensor-actuator devices
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7758)
AUTHORS Siciliano V.
TITLE Direct Submission
JOURNAL Submitted (22-MAR-2018) Biological Engineering, Massachusetts
Institute of Technology, 500 Tech Square, Cambridge, MA 02139, USA
REFERENCE 3 (bases 1 to 7758)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7758)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(22-MAR-2018) Biological Engineering, Massachusetts Institute of
Technology, 500 Tech Square, Cambridge, MA 02139, USA"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7758
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(222..810)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(984..1841)
/label="AmpR"
/note="beta-lactamase"
promoter complement(1842..1946)
/label="AmpR promoter"
rep_origin complement(1972..2427)
/direction=LEFT
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 2569..2585
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 2595..2613
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 2628..3431
/label="HA-L"
/note="left homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
misc_feature 3510..3535
/label="SA"
/note="splice acceptor site"
protein_bind 4207..4227
/label="attB4"
/note="core recombination site for the Gateway(R) BP
reaction"
promoter 4252..5424
/label="EF-1-alpha promoter"
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
protein_bind 5446..5470
/label="attB1"
/note="recombination site for the Gateway(R) BP reaction"
CDS 5484..5741
/gene="tat"
/label="Protein Tat"
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate HXB2). Accession#: P04608"
protein_bind complement(5791..5815)
/label="attB2"
/note="recombination site for the Gateway(R) BP reaction"
polyA_signal 5970..6025
/label="beta-globin poly(A) signal"
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(6386..6402)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 6410..6426
/label="lac operator"
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6434..6464)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 6479..6500
/label="CAP binding site"
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 6673..7509
/label="HA-R"
/note="right homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
promoter complement(7539..7557)
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(7578..7594)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(7602..7618)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7626..7656)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(7671..7692)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
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