Basic Vector Information
- Vector Name:
- pEXPR16
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8052 bp
- Type:
- Mammalian expression vector
- Replication origin:
- ori
- Source/Author:
- Siciliano V.
- Promoter:
- EF-1α
pEXPR16 vector Map
pEXPR16 vector Sequence
LOCUS 40924_18851 8052 bp DNA circular SYN 18-DEC-2018
DEFINITION Mammalian expression vector pEXPR16, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8052)
AUTHORS Siciliano V.
TITLE Engineering modular intracellular protein sensor-actuator devices
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8052)
AUTHORS Siciliano V.
TITLE Direct Submission
JOURNAL Submitted (22-MAR-2018) Biological Engineering, Massachusetts
Institute of Technology, 500 Tech Square, Cambridge, MA 02139, USA
REFERENCE 3 (bases 1 to 8052)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8052)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(22-MAR-2018) Biological Engineering, Massachusetts Institute of
Technology, 500 Tech Square, Cambridge, MA 02139, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8052
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(222..810)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(984..1841)
/label=AmpR
/note="beta-lactamase"
promoter complement(1842..1946)
/label=AmpR promoter
rep_origin complement(1972..2427)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 2569..2585
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 2595..2613
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 2628..3431
/label=HA-L
/note="left homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
misc_feature 3510..3535
/label=SA
/note="splice acceptor site"
protein_bind 4207..4227
/label=attB4
/note="core recombination site for the Gateway(R) BP
reaction"
promoter 4252..5424
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
protein_bind 5446..5470
/label=attB1
/note="recombination site for the Gateway(R) BP reaction"
regulatory 5473..5482
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 5479..6084
/codon_start=1
/product="nNS3"
/label=nNS3
/note="derived from HCV"
/protein_id="AVV68396.1"
/translation="MAPIGSVVIVGRIILSGRGGPITAYSQQTRGLLGCIITSLTGRDK
NQVEGEVQVVSTATQSFLATCVNGACWTVFHGAGSKTLAGPKGPITQMYTNVDQDLVGW
PAPPGARSLTPCTCGSSDLYLVTRHADVIPVRRRGDTRGSLLSPRPISYLKGSSGGPLL
CPSGHVVGIFRAAVCTRGVAKAVDFIPVESMETTMRGS"
protein_bind complement(6085..6109)
/label=attB2
/note="recombination site for the Gateway(R) BP reaction"
polyA_signal 6264..6319
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(6680..6696)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 6704..6720
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6728..6758)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6773..6794
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 6967..7803
/label=HA-R
/note="right homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
promoter complement(7833..7851)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(7872..7888)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(7896..7912)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7920..7950)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(7965..7986)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
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