pETL7 vector (V006455)

LOCUS       V006455                 5246 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006455
VERSION     V006455
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Koksharov MI, Ugarova NN.
  TITLE     Thermostabilization of luciferase by in vivo directed evolution
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Koksharov MI, Ugarova NN.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2010) Department of Chemistry, Lomonosov Moscow
            State University, Leninskie Gory, Bld. 1/11, GSP-3, Moscow 119991,
            Russia
REFERENCE   3  (bases 1 to 5246)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5246)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-JUL-2010) Department of Chemistry, Lomonosov Moscow State
            University, Leninskie Gory, Bld. 1/11, GSP-3, Moscow 119991, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..39
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             71..93
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             113..1750
                     /note="Luciferin 4-monooxygenase from Luciola mingrelica.
                     Accession#: Q26304"
                     /label="Luciferin 4-monooxygenase"
     CDS             1763..1780
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      1847..1894
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      1931..2386
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2413..2517
                     /label="AmpR promoter"
     CDS             2518..3375
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3549..4137
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(4323..4465)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(4570..4758)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"