pET-T7p(-3G)-SpacerA-GFP-LVA vector (V006493)

LOCUS       40924_18286        4230 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Synthetic vector pET-T7p(-3G)-SpacerA-GFP-LVA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Schaerli Y, Gili M, Isalan M.
  TITLE     A split intein T7 RNA polymerase for transcriptional AND-logic
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25262348
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Schaerli Y, Gili M, Isalan M.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2014) Centre for Genomic Regulation (CRG), 
            EMBL/CRG Systems Biology Research Unit, Dr. Aiguader 88, Barcelona 
            08003, Spain
REFERENCE   3  (bases 1 to 4230)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4230)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JUL-2014) Centre for Genomic Regulation (CRG), EMBL/CRG Systems 
            Biology Research Unit, Dr. Aiguader 88, Barcelona 08003, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             495..683
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    788..930
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1116..1704)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1878..2735)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2736..2840)
                     /label=AmpR promoter
     primer_bind     2880..2896
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2909..2926
                     /gene="GFP-LVA"
                     /label=T7(-3G) promoter
                     /note="T7(-3G) promoter"
                     /regulatory_class="promoter"
     misc_feature    2927..2960
                     /gene="GFP-LVA"
                     /label=spacerA
                     /note="spacerA"
     RBS             3068..3090
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3098..3811
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     terminator      3961..4008
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4017..4103
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4195..4222
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"