pET-hsp12 vector (V006508)

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LOCUS       V006508                 5898 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006508
VERSION     V006508
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5898)
  AUTHORS   Leger A, Hocquellet A, Dieryck W, Moine V, Marchal A, Marullo P,
            Josseaume A, Cabanne C.
  TITLE     Production and purification of the native Saccharomyces cerevisiae
            Hsp12 in Escherichia coli
  JOURNAL   J. Agric. Food Chem. (2017) In press
   PUBMED   28871789
REFERENCE   2  (bases 1 to 5898)
  AUTHORS   Leger A, Hocquelet A, Dieryck W, Moine V, Marchal A, Josseaume A,
            Cabanne C.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2017) ENSTBB, Institut Polytechnique de Bordeaux,
            146 rue Leo Saignat, Bordeaux, Gironde 33076, France
REFERENCE   3  (bases 1 to 5898)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5898)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Agric.
            Food Chem. (2017) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-APR-2017) ENSTBB, Institut Polytechnique de Bordeaux, 146 rue
            Leo Saignat, Bordeaux, Gironde 33076, France"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(585..1442)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1443..1535)
                     /label="AmpR promoter"
     rep_origin      1616..2204
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2390..2532)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2637..2825)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(3363..3384)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3400..4479)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(4480..4557)
                     /label="lacI promoter"
     promoter        4870..4888
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4889..4913
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4928..4950
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4958..5284
                     /gene="HSP12"
                     /label="12 kDa heat shock protein"
                     /note="12 kDa heat shock protein from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P22943"
     CDS             5331..5363
                     /label="HSV tag"
                     /note="HSV (herpes simplex virus) epitope tag"
     CDS             5370..5387
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      5553..5639
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      5731..5758
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      5826..5873
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"