pERV3 vector (V006528)

MSDS

Basic Vector Information

Vector Name:: pERV3

Antibiotic Resistance:: Kanamycin

Length:: 8363 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Vaillancourt P, Grafsky AJ.

Promoter:: CMV

pERV3 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pERV3 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V006528                 8363 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006528
VERSION     V006528
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Vaillancourt P, Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-1998) Technical Services, Stratagene, 11011 N.
            Torrey Pines Road, La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 8363)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8363)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (13-OCT-1998) Technical Services, Stratagene, 11011 N. Torrey Pines
            Road, La Jolla, CA 92037, USA"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..462
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    complement(468..600)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     intron          complement(759..824)
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(912..2297)
                     /gene="RXRA"
                     /label="Retinoic acid receptor RXR-alpha"
                     /note="Retinoic acid receptor RXR-alpha from Homo sapiens.
                     Accession#: P19793"
     CDS             complement(2295..2324)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     misc_feature    complement(2324..2902)
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             complement(2910..4760)
                     /codon_start=1
                     /product="ecdysone receptor VgEcR"
                     /label="ecdysone receptor VgEcR"
                     /protein_id="AAC95155.1"
                     /translation="MLGERKDRASGYHYNALTCGSCKVFFRRSVTKSAVYCCKFGRACE
                     MDMYMRRKCQECRLKKCLAVGMRPECVVPENQCAMKRREKKAQKEKDKMTTSPSSQHGG
                     NGSLASGGGQDFVKKEILDLMTCEPPQHATIPLLPDEILAKCQARNIPSLTYNQLAVIY
                     KLIWYQDGYEQPSEEDLRRIMSQPDENESQTDVSFRHITEITILTVQLIVEFAKGLPAF
                     TKIPQEDQITLLKACSSEVMMLRMARRYDHSSDSIFFANNRSYTRDSYKMAGMADNIED
                     LLHFCRQMFSMKVDNVEYALLTAIVIFSDRPGLEKAQLVEAIQSYYIDTLRIYILNRHC
                     GDSMSLVFYAKLLSILTELRTLGNQNAEMCFSLKLKNRKLPKFLEEIWDVHAIPPSVQS
                     HLQITQEENERLERAERMRASVGGAITAGIDCDSASTSAAAAAAQHQPQPQPQPQPSSL
                     TQNDSQHQTQPQLQPQLPPQLQGQLQPQLHPQLQTQLQPQIQPQPQLLPVSAPVPASVT
                     APGSLSAVSTSSEYMGGSAAIGPITPATTSSITAAVTASSTTSAVPMGNGVGVGVGVGG
                     NVSMYANAQTAMALMGVALHSHQEQLIGGVAVKSEHSTTA"
     CDS             complement(4844..5077)
                     /label="VP16 AD"
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et
                     al., 1989)"
     CDS             complement(5078..5107)
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     regulatory      5107..5116
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5153..5356)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(5357..5660)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(5855..6443)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     polyA_signal    complement(6772..6819)
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(7054..7845)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(7880..8237)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        complement(8239..8343)
                     /label="AmpR promoter"

This page is informational only.