Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V006530 | pEPI-1 | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
The pEPI-1 vector contains two mammalian transcription units and a total of 305 CpG islands, which are located predominantly within the vector elements necessary for bacterial propagation and known to be counterproductive for persistent long-term transgene expression. The episomal replication of vector pEPI-1 depends on a transcription unit starting from the constitutively expressed Cytomegalovirus immediate early promoter (CMV-IEP) and directed into a 2000 bp long matrix attachment region sequence (MARS) derived from the human β-interferon gene.
- Vector Name:
- pEPI-1
- Antibiotic Resistance:
- Kanamycin
- Length:
- 6944 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Zhang X, Wang XY, Jia YL, Guo X, Wang YF, Wang TY.
- Growth Strain(s):
- DH10b
- Growth Temperature:
- 37℃
pEPI-1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
References
- Piechaczek C, Fetzer C, Baiker A, Bode J, Lipps HJ. A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells. Nucleic Acids Res. 1999;27(2):426-428. doi:10.1093/nar/27.2.426
pEPI-1 vector Sequence
LOCUS pEPI-1. 6944 bp DNA circular SYN 14-JUN-2022
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS pEPI-1.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6944)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6944
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 1..304
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 305..508
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 553..1269
/label=EGFP
/note="enhanced GFP"
misc_feature 1276..3476
/label=S/MAR
/note="S/MAR"
/note="S/MARs-element(Scaffold or Matrix Attachment
Regions) are typically 70% A/T-rich sequences and are often
found in association with chromosomal origins of
bidirectional replication. It could be used to construct
non-viral episomal vectors in mammalian cells."
misc_feature 3483..3548
/label=MCS
/note="multiple cloning site"
polyA_signal 3672..3793
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3800..4255)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4282..4386
/label=AmpR promoter
promoter 4388..4745
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 4780..5571
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
polyA_signal 5806..5853
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 6182..6770
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 6885..6944
/label=CMV
/note="Human cytomegalovirus immediate early
enhancer/promoter"