pENTR223-MPG vector (V006536)

LOCUS       V006536                 3669 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006536
VERSION     V006536
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Rual JF, Hirozane-Kishikawa T, Hao T, Bertin N, Li S, Dricot A, Li
            N, Rosenberg J, Lamesch P, Vidalain PO, Clingingsmith TR, Hartley
            JL, Esposito D, Cheo D, Moore T, Simmons B, Sequerra R, Bosak S,
            Doucette-Stamm L, Le Peuch C, Vandenhaute J, Cusick ME, Albala JS,
            Hill DE, Vidal M.
  TITLE     Human ORFeome version 1.1: a platform for reverse proteomics
  JOURNAL   Genome Res. 14 (10B), 2128-2135 (2004)
   PUBMED   15489335
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3669)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   4  (bases 1 to 3669)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3669)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            14 (10B), 2128-2135 (2004)"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (06-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(88..115)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(207..293)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     357..373
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    389..486
                     /label="attL2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             509..1366
                     /gene="MPG"
                     /label="DNA-3-methyladenine glycosylase"
                     /note="DNA-3-methyladenine glycosylase from Homo sapiens.
                     Accession#: P29372"
     protein_bind    complement(1369..1467)
                     /label="attL2"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1485..1503)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1508..1524)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1955..2743
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      2839..3427
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"