pHC60 vector (V005592)

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LOCUS       V005592                10513 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005592
VERSION     V005592
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Untergasser A, Geurts R, Franssen H.
  TITLE     pHC60 - a very stable plasmid for fluorescence mircroscopy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10513)
  AUTHORS   Untergasser A, Geurts R, Franssen H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2008) Laboratory of Molecular Biology, Wageningen
            University, Dreijenlaan 3, Wageningen 6703HA, The Netherlands
REFERENCE   3  (bases 1 to 10513)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10513)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-AUG-2008) Laboratory of Molecular Biology, Wageningen
            University, Dreijenlaan 3, Wageningen 6703HA, The Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     508..524
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    525..581
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     promoter        613..631
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             663..685
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             693..1409
                     /label="Cycle 3 GFP"
                     /note="Cycle 3 GFP (Crameri et al., 1996)"
     terminator      1474..1521
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     primer_bind     complement(1581..1597)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    1605..1621
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1629..1659)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1674..1695)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3178..3888)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
     oriT            4033..4142
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             4175..4543
                     /label="traJ"
                     /note="oriT-recognizing protein"
     CDS             7240..7488
                     /gene="parD"
                     /label="Antitoxin ParD"
                     /note="Antitoxin ParD from Escherichia coli. Accession#:
                     P22995"
     CDS             7488..7796
                     /gene="parE"
                     /label="Toxin ParE"
                     /note="Toxin ParE from Escherichia coli. Accession#:
                     Q79EC5"
     CDS             complement(8093..9172)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(9173..9250)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             complement(9312..10508)
                     /label="TcR"
                     /note="tetracycline efflux protein"