pHB518 vector (V005613)

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LOCUS       V005613                 4911 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005613
VERSION     V005613
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Gao H, Xu X.
  TITLE     pHB518
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Gao H, Xu X.
  TITLE     unpublished
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4911)
  AUTHORS   Gao H, Xu X.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2007) Institute of Hydrobiology, Chinese Academy
            of Science, #7 Donghu South Rd, Wuchang, Wuhan, Hubei 430072, China
REFERENCE   4  (bases 1 to 4911)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4911)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (20-JUN-2007) Institute of Hydrobiology, Chinese Academy of Science,
            #7 Donghu South Rd, Wuchang, Wuhan, Hubei 430072, China"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             445..1176
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     CDS             complement(1317..1973)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(1974..2076)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             complement(2267..3079)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(3226..3242)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3716..3820
                     /label="AmpR promoter"
     rep_origin      4141..4729
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"