Basic Vector Information
- Vector Name:
- pHapII
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6838 bp
- Type:
- GFP expression vector
- Replication origin:
- ori
- Source/Author:
- Zhang N, Wu K, He X, Li S-Q., Zhang Z-H., Shen B, Yang Y-M., Zhang R-F., Huang Q-W., Shen Q-R.
pHapII vector Vector Map
pHapII vector Sequence
LOCUS 40924_24114 6838 bp DNA circular SYN 18-DEC-2018 DEFINITION GFP expression vector pHapII, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6838) AUTHORS Zhang N, Wu K, He X, Li S-Q., Zhang Z-H., Shen B, Yang Y-M., Zhang R-F., Huang Q-W., Shen Q-R. TITLE A new bioorganic fertilizer can effectively control banana wilt by strong colonization with Bacillus subtilis N11 JOURNAL Plant Soil 344 (1-2), 87-97 (2011) REFERENCE 2 (bases 1 to 6838) AUTHORS Zhang Z, Tian W, Liu D. TITLE Development of a GFP vector system in Bacillus under the control of the constitutive promoter HpaII JOURNAL Unpublished REFERENCE 3 (bases 1 to 6838) AUTHORS Zhang Z, Tian W, Liu D, Zhou T, Wu Y, Liu Y, Xu L. TITLE Direct Submission JOURNAL Submitted (26-APR-2010) Jiang Su Provincial Key Lab for Organic Solid Waste Utilization, Nanjing Agricultural University, Wei Gang #1, Nan jing, Jiang Su 210095, China REFERENCE 4 (bases 1 to 6838) TITLE Direct Submission REFERENCE 5 (bases 1 to 6838) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant Soil 344 (1-2), 87-97 (2011)" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted (26-APR-2010) Jiang Su Provincial Key Lab for Organic Solid Waste Utilization, Nanjing Agricultural University, Wei Gang #1, Nan jing, Jiang Su 210095, China" COMMENT SGRef: number: 4; type: "Journal Article" FEATURES Location/Qualifiers source 1..6838 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" CDS 814..1815 /codon_start=1 /label=repB /note="RepB replication protein" /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE" CDS 1987..2748 /codon_start=1 /product="kanamycin nucleotidyltranferase" /label=kanamycin nucleotidyltranferase /note="KM" /protein_id="ADK73494.1" /translation="MNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGRQTD GPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLTHGQFF SILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQGPTTFLP SLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSGQLSDSEK LLESLENFWNGIQEWTERHGYIVDVSKRIPF" CDS 2965..3216 /codon_start=1 /product="ble" /label=ble /protein_id="ADK73495.1" /translation="MRMLQSIPALPVGDIKKSIGFYWDKVGFTLVHHEDGFAVLMCNEV RIHLWEASDEGWRLVVMIHRFVQVRSRLLLVLLVAALK" CDS 3873..4586 /codon_start=1 /label=yeGFP /note="yeast-enhanced green fluorescent protein" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" primer_bind complement(4617..4633) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(4641..4657) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4665..4695) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(4710..4731) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(5019..5607) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5781..6638) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(6639..6743) /label=AmpR promoter
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