pGX1 vector (V005627)

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LOCUS       V005627                 7504 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005627
VERSION     V005627
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7504)
  AUTHORS   Ji M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2015) Shanghai Advanced Research Institute, CAS,
            Haike Road 99, Shanghai 201210, China
REFERENCE   2  (bases 1 to 7504)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7504)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (16-DEC-2015) Shanghai Advanced Research Institute, CAS, Haike Road
            99, Shanghai 201210, China"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             746..760
                     /label="enterokinase site"
                     /note="enterokinase recognition and cleavage site"
     CDS             complement(1499..2356)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2357..2461)
                     /label="AmpR promoter"
     primer_bind     2935..2951
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      2952..3129
                     /label="xylA promoter"
                     /note="xylA promoter"
                     /regulatory_class="promoter"
     CDS             3128..3841
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     primer_bind     complement(3881..3897)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3905..3921)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3929..3959)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3974..3995)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4283..4871)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5074..5721)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"