Basic Vector Information
- Vector Name:
- pGWB401
- Antibiotic Resistance:
- Streptomycin
- Length:
- 10844 bp
- Type:
- Gateway binary vector
- Replication origin:
- ori
- Host:
- Plants
- Source/Author:
- Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, Ishiguro S.
- Promoter:
- NOS
pGWB401 vector Map
pGWB401 vector Sequence
LOCUS 40924_22834 10844 bp DNA circular SYN 18-DEC-2018
DEFINITION Gateway binary vector pGWB401 DNA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10844)
AUTHORS Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata
R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T,
Ishiguro S.
TITLE Improved Gateway Binary Vectors: High-Performance Vectors for
Creation of Fusion Constructs in Transgenic Analysis of Plants
JOURNAL Biosci. Biotechnol. Biochem. (2007) In press
PUBMED 17690442
REFERENCE 2 (bases 1 to 10844)
AUTHORS Nakagawa T, Ishiguro S.
TITLE Direct Submission
JOURNAL Submitted (21-FEB-2007) Tsuyoshi Nakagawa, Shimane University,
Center for Integrated Research in Science; 1060 Nishikawatsu,
Matsue, Shimane 690-8504, Japan
(E-mail:tnakagaw@life.shimane-u.ac.jp, Tel:81-852-32-6595,
Fax:81-852-32-6109)
REFERENCE 3 (bases 1 to 10844)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 10844)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Biosci.
Biotechnol. Biochem. (2007) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(21-FEB-2007) Tsuyoshi Nakagawa, Shimane University, Center for
Integrated Research in Science"; volume: " 1060 Nishikawatsu,
Matsue, Shimane 690-8504, Japan
(E-mail:tnakagaw@life.shimane-u.ac.jp, Tel:81-852-32-6595, Fax";
pages: "81-852-32-6109"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT constructed using pPZP221.
FEATURES Location/Qualifiers
source 1..10844
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature complement(54..78)
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
primer_bind 281..297
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 338..462
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
promoter 499..529
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
CDS 583..1239
/codon_start=1
/label=CmR
/note="chloramphenicol acetyltransferase"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
CDS 1584..1886
/codon_start=1
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
protein_bind complement(1930..2054)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
terminator 2086..2338
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(2372..2388)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 2396..2412
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2420..2450)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(2465..2486)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
terminator complement(2520..2772)
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(3165..3953)
/codon_start=1
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase from Tn5"
/translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
promoter complement(3977..4160)
/label=NOS promoter
/note="nopaline synthase promoter"
misc_feature complement(4366..4390)
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 4911..5699
/codon_start=1
/label=SmR
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 5948..6536
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(6722..6862)
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(7206..7400)
/direction=LEFT
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(7469..8539)
/codon_start=1
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
CDS complement(8971..9597)
/codon_start=1
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
This page is informational only.