pGUG vector (V005866)

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LOCUS       40924_22794        5199 bp DNA     circular SYN 18-DEC-2018
DEFINITION  GFP fusion vector pGUG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Oka A, Sugisaki H, Takanami M.
  TITLE     Nucleotide sequence of the kanamycin resistance transposon Tn903
  JOURNAL   J. Mol. Biol. 147 (2), 217-226 (1981)
  PUBMED    6270337
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Heim R, Cubitt AB, Tsien RY.
  TITLE     Improved green fluorescence
  JOURNAL   Nature 373 (6516), 663-664 (1995)
  PUBMED    7854443
REFERENCE   3  (bases 1 to 5199)
  AUTHORS   Wach A, Brachat A, Alberti-Segui C, Rebischung C, Philippsen P.
  TITLE     Heterologous HIS3 marker and GFP reporter modules for PCR-targeting 
            in Saccharomyces cerevisiae
  JOURNAL   Yeast 13 (11), 1065-1075 (1997)
  PUBMED    9290211
REFERENCE   4  (bases 1 to 5199)
  AUTHORS   Wendland J, Ayad-Durieux Y, Knechtle P, Rebischung C, Philippsen P.
  TITLE     PCR-based gene targeting in the filamentous fungus Ashbya gossypii
  JOURNAL   Gene 242 (1-2), 381-391 (2000)
  PUBMED    10721732
REFERENCE   5  (bases 1 to 5199)
  AUTHORS   Knechtle P, Dietrich F, Philippsen P.
  TITLE     Maximal Polar Growth Potential Depends on the Polarisome Component 
            AgSpa2 in the Filamentous Fungus Ashbya gossypii
  JOURNAL   Mol. Biol. Cell 14 (10), 4140-4154 (2003)
  PUBMED    12937275
REFERENCE   6  (bases 1 to 5199)
  AUTHORS   Knechtle P, Philippsen P.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2002) Microbiology, Biozentrum, Universitaet 
            Basel, Klingelbergstrasse 70, Basel 4056, Switzerland
REFERENCE   7  (bases 1 to 5199)
  TITLE     Direct Submission
REFERENCE   8  (bases 1 to 5199)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Mol. 
            Biol."; date: "1981"; volume: "147"; issue: "2"; pages: "217-226"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Nature"; 
            date: "1995"; volume: "373"; issue: "6516"; pages: "663-664"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Yeast"; 
            date: "1997"; volume: "13"; issue: "11"; pages: "1065-1075"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Gene 242 
            (1-2), 381-391 (2000)"
COMMENT     SGRef: number: 5; type: "Journal Article"; journalName: "Mol. Biol. 
            Cell"; date: "2003"; volume: "14"; issue: "10"; pages: "4140-4154"
COMMENT     SGRef: number: 6; type: "Journal Article"; journalName: "Submitted 
            (24-MAY-2002) Microbiology, Biozentrum, Universitaet Basel, 
            Klingelbergstrasse 70, Basel 4056, Switzerland"
COMMENT     SGRef: number: 7; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        184..202
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    257..292
                     /label=encodes 6x Gly-Ala linker
                     /note="encodes 6x Gly-Ala linker"
     CDS             305..1015
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
     regulatory      1027..1268
                     /label=Saccharomyces cerevisiae URA3
                     /note="Saccharomyces cerevisiae URA3"
                     /regulatory_class="terminator"
     regulatory      1275..1881
                     /label=Saccharomyces cerevisiae TEF2
                     /note="Saccharomyces cerevisiae TEF2"
                     /regulatory_class="promoter"
     CDS             1882..2688
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      2692..2966
                     /label=Saccharomyces cerevisiae TEF2
                     /note="Saccharomyces cerevisiae TEF2"
                     /regulatory_class="terminator"
     promoter        complement(3037..3055)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3313..3901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4075..4932)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4933..5037)
                     /label=AmpR promoter