pGT1 vector (V005869)

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LOCUS       40924_22774       11207 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Dual-tagging gene trap vector pGT1 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11207)
  AUTHORS   Lukacsovich T, Asztalos Z, Awano W, Baba K, Kondo S, Niwa S, 
            Yamamoto D.
  TITLE     Dual-tagging gene trap of novel genes in Drosophila melanogaster
  JOURNAL   Genetics 157 (2), 727-742 (2001)
  PUBMED    11156992
REFERENCE   2  (bases 1 to 11207)
  AUTHORS   Lukacsovich T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-1999) Tamas Lukacsovich, University of California,
            Irvine, Developmental and Cell Biology; Room 4444, McGaugh Hall, 
            Irvine, California 92697-2300, USA (E-mail:tlukacs@uci.edu, 
            Tel:1-949-824-3226, Fax:1-949-824-3571)
REFERENCE   3  (bases 1 to 11207)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11207)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "2001"; volume: "157"; issue: "2"; pages: "727-742"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAY-1999) Tamas Lukacsovich, University of California, Irvine, 
            Developmental and Cell Biology"; volume: " Room 4444, McGaugh Hall, 
            Irvine, California 92697-2300, USA (E-mail:tlukacs@uci.edu, 
            Tel:1-949-824-3226, Fax"; pages: "1-949-824-3571"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          5409..8290
                     /mol_type="other DNA"
                     /db_xref="taxon:7227"
                     /organism="Drosophila melanogaster"
     misc_feature    complement(1..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     CDS             275..2917
                     /label=GAL4
                     /note="GAL4 transcriptional activator"
     CDS             complement(3627..4418)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4461..4909)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             join(5409..5480,5846..6146,6221..6875,6908..7253,7474..7605,
                     7676..8290)
                     /codon_start=1
                     /gene="mini-white"
                     /product="white protein"
                     /label=mini-white
                     /protein_id="BAA78210.1"
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNLIFEIPYHCRVTADASQSCIN
                     QGFGQAKNYGTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRT
                     RGLFCNERHIPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVS
                     PSGMRLLNGQPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVA
                     RVDQVIQELSLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLD
                     SFTAHSVVQVLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDF
                     FSYITLHLNSYPAWVPSVLPTTIRRTFTYRCWPLCPDGRSSPVIGSPRYGDNFAISKVA
                     RDMEQLLATKNLEKPLEQPENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTT
                     MVAILIGLIFLGQQLTQVGVMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARS
                     RLYRCDTYFLGKTIAELPLFLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVST
                     SFGYLISCASSSTSMALSVGPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEG
                     LLINQWADVEPGEISCTSSNTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLA
                     YLALRLRARRKE"
     gene            join(5409..5480,5846..6146,6221..6875,6908..7253,7474..7605,
                     7676..8290)
                     /gene="mini-white"
                     /label=mini-white
     rep_origin      complement(8682..9270)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9444..10301)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10302..10406)
                     /label=AmpR promoter