pGrDL_SPb vector (V005892)

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LOCUS       40924_22573        7769 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGrDL_SPb, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Moyle RL, Carvalhais L, Nowak E, Pretorius L-S., Subramaniam G, 
            Dalton-Morgan J, Schenk PM.
  TITLE     Quantifying MicroRNA Directed Repression of Targeted Transcripts 
            using an Optimised Transient Dual Luciferase Assay
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   Moyle RL, Carvalhais L, Nowak E, Pretorius L-S., Subramaniam G, 
            Dalton-Morgan J, Schenk PM.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2016) School of Agriculture and Food Science, 
            University of Queensland, St Lucia, Brisbane, QLD 4072, Australia
REFERENCE   3  (bases 1 to 7769)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7769)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-AUG-2016) School of Agriculture and Food Science, University of 
            Queensland, St Lucia, Brisbane, QLD 4072, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    542..564
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     terminator      complement(587..839)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(857..1789)
                     /label=Rluc
                     /note="luciferase from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
     regulatory      complement(1805..2811)
                     /label=tomato Act7 promoter
                     /note="tomato Act7 promoter"
                     /regulatory_class="promoter"
     regulatory      2324..2715
                     /label=CaMV 35S promoter
                     /note="CaMV 35S promoter"
                     /regulatory_class="promoter"
     primer_bind     2936..2952
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2962..2980
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3055..3398
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3405..5054
                     /label=luciferase
                     /note="firefly luciferase"
     regulatory      5167..5332
                     /label=35S terminator
                     /note="35S terminator"
                     /regulatory_class="terminator"
     promoter        complement(5368..5386)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5407..5423)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5431..5447)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5455..5486)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5501..5522)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    5761..5785
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(5876..6464)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6638..7450)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      7741..7769
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"