pGPI-SceI-2 vector (V005913)

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LOCUS       40924_22478        4925 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mutagenesis vector pGPI-SceI-2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Hamad MA, Di Lorenzo F, Molinaro A, Valvano MA.
  TITLE     Aminoarabinose is essential for lipopolysaccharide export and 
            intrinsic antimicrobial peptide resistance in Burkholderia 
            cenocepacia(dagger)
  JOURNAL   Mol. Microbiol. 85 (5), 962-974 (2012)
  PUBMED    22742453
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Hamad MA, Di Lorenzo F, Molinaro A, Valvano MA.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2012) Microbiology and Immunology, Western 
            University, 1151 Richmond Street North, London, Ontario N6A 5C1, 
            Canada
REFERENCE   3  (bases 1 to 4925)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4925)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. 
            Microbiol."; date: "2012"; volume: "85"; issue: "5"; pages: 
            "962-974"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-DEC-2012) Microbiology and Immunology, Western University, 1151 
            Richmond Street North, London, Ontario N6A 5C1, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            626..735
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             768..1136
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     CDS             complement(1102..1527)
                     /codon_start=1
                     /product="TraI"
                     /label=TraI
                     /protein_id="AGC79936.1"
                     /translation="MNVQVVGVVMHGTDALMLGEAKPSADAVLNRAQRLRVGLLSRAEA
                     NQQVIGLVGLGTGVAVLGRHHLGHDSGQGVCLAVRDAHVTQALGLALLVGDVLHQLREV
                     ALLDGAHGDVLGNHAHPPAVLAAKKVMALPSGGPRPS"
     rep_origin      1757..2145
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     misc_feature    2149..2175
                     /label=multiple cloning site 1
                     /note="multiple cloning site 1"
     misc_feature    2388..2421
                     /label=multiple cloning site 2
                     /note="multiple cloning site 2"
     promoter        3150..3178
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3504..3737
                     /codon_start=1
                     /label=TpR
                     /note="E. coli plasmid-associated dihydrofolate reductase"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     misc_feature    3743..3760
                     /label=I SceI recognition site
                     /note="I SceI recognition site"