Basic Vector Information
- Vector Name:
- pGloSensor-20F cAMP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6990 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Stecha PF, McNamara B, Binkowski BF.
- Promoter:
- SV40
pGloSensor-20F cAMP vector Map
pGloSensor-20F cAMP vector Sequence
LOCUS 40924_22408 6990 bp DNA circular SYN 18-DEC-2018 DEFINITION Expression vector pGloSensor-20F cAMP, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6990) AUTHORS Stecha PF, McNamara B, Binkowski BF. TITLE Direct Submission JOURNAL Submitted (29-MAY-2008) Scientific Communications, Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711-5399, USA REFERENCE 2 (bases 1 to 6990) TITLE Direct Submission REFERENCE 3 (bases 1 to 6990) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (29-MAY-2008) Scientific Communications, Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711-5399, USA" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..6990 /mol_type="other DNA" /organism="synthetic DNA construct" CDS complement(8..865) /label=AmpR /note="beta-lactamase" polyA_signal 970..1018 /label=poly(A) signal /note="synthetic polyadenylation signal" misc_feature 1032..1123 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" enhancer 1275..1653 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1654..1857 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 1890..1908 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1922..4027 /codon_start=1 /product="GloSensor cAMP" /label=GloSensor cAMP /protein_id="ACF75745.1" /translation="MPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYV NNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQH PNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVD EVPKGLTGKLDARKIREILIKAKKGSSGGSGGSGGGMYESFIESLPFLKSLEFSERLKV VDVIGTKVYNDGEQIIAQGDSADSFFIVESGEVKITMKRKGKSEVEENGAVEIARCSRG QYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQLVAL FGTNMDIVAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYA EYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERE LLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHL PPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQI IPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPT LFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAI LITPEGV" polyA_signal complement(4069..4190) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter 4384..4741 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 4772..5806 /label=HygR /note="hygromycin B phosphotransferase" misc_feature 5833..5881 /note="synthetic poly(A) signal/transcriptional pause site" polyA_signal 5833..5881 /label=synthetic polyadenylation signal /note="synthetic polyadenylation signal" primer_bind 5948..5967 /label=RVprimer4 /note="RVprimer4" rep_origin complement(6208..6795) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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