Basic Vector Information
- Vector Name:
- pGL3hEcadP-mE2box1-3
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5011 bp
- Type:
- Reporter vector
- Replication origin:
- ori
- Source/Author:
- De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.
pGL3hEcadP-mE2box1-3 vector Map
pGL3hEcadP-mE2box1-3 vector Sequence
LOCUS 40924_22048 5011 bp DNA circular SYN 18-DEC-2018 DEFINITION Reporter vector pGL3hEcadP-mE2box1-3, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5011) AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished REFERENCE 2 (bases 1 to 5011) AUTHORS De Schamphelaire W. TITLE Direct Submission JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM REFERENCE 3 (bases 1 to 5011) TITLE Direct Submission REFERENCE 4 (bases 1 to 5011) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5011 /mol_type="other DNA" /organism="synthetic DNA construct" regulatory 7..228 /label=hEcadm /note="hEcadm" /regulatory_class="promoter" CDS 281..1930 /label=luciferase /note="firefly luciferase" polyA_signal complement(1974..2095) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(2514..3102) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3276..4133) /label=AmpR /note="beta-lactamase" promoter complement(4134..4238) /label=AmpR promoter rep_origin 4265..4720 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" polyA_signal 4851..4899 /label=poly(A) signal /note="synthetic polyadenylation signal" misc_feature 4913..5004 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene"
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