Basic Vector Information
- Vector Name:
- pGL3casp11pd3
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5065 bp
- Type:
- Reporter vector
- Replication origin:
- ori
- Source/Author:
- De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.
pGL3casp11pd3 vector Map
pGL3casp11pd3 vector Sequence
LOCUS 40924_22043 5065 bp DNA circular SYN 18-DEC-2018 DEFINITION Reporter vector pGL3casp11pd3, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5065) AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished REFERENCE 2 (bases 1 to 5065) AUTHORS De Schamphelaire W. TITLE Direct Submission JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM REFERENCE 3 (bases 1 to 5065) TITLE Direct Submission REFERENCE 4 (bases 1 to 5065) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5065 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 29..64 /label=Adaptor sequence /note="Adaptor sequence" regulatory 65..275 /note="186 bp mCASP-11 promoter fragm." /regulatory_class="promoter" CDS 335..1984 /label=luciferase /note="firefly luciferase" polyA_signal complement(2028..2149) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(2568..3156) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3330..4187) /label=AmpR /note="beta-lactamase" promoter complement(4188..4292) /label=AmpR promoter rep_origin 4319..4774 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" polyA_signal 4905..4953 /label=poly(A) signal /note="synthetic polyadenylation signal" misc_feature 4967..5058 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene"
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