pGL3-MSTN-3.8kb vector (V005967)

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LOCUS       40924_21973        8596 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pGL3-MSTN-3.8kb, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Bi Y, Qiao X, Hua Z, Zhang L, Liu X, Li L, Hua W, Xiao H, Zhou J, 
            Wei Q, Zheng X.
  TITLE     An asymmetric PCR-based, reliable and rapid single-tube native DNA 
            engineering strategy
  JOURNAL   BMC Biotechnol. 12, 39 (2012)
  PUBMED    22768962
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   Bi Y, Zheng X, Qiao X, Zhang R, Jiang L, Ouyang H, Shao C, Hua Z, 
            Zhou J, Liu X, Li L, Xiao H, Hua W, Zhang L, Wei Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2011) Biotechnology Group, Institute of Animal 
            Science and Veterinary, Hubei Academy of Agricultural Science, No. 
            1, Nanhu Yaoyuan, Hongshan District, Wuhan, Hubei 430064, P. R. 
            China
REFERENCE   3  (bases 1 to 8596)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8596)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol. 12, 39 (2012)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-AUG-2011) Biotechnology Group, Institute of Animal Science and 
            Veterinary, Hubei Academy of Agricultural Science, No. 1, Nanhu 
            Yaoyuan, Hongshan District, Wuhan, Hubei 430064, P. R. China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      61..3838
                     /label=porcine MSTN promoter
                     /note="porcine MSTN promoter"
                     /regulatory_class="promoter"
     regulatory      3707..3711
                     /regulatory_class="CAAT_signal"
     regulatory      3748..3754
                     /regulatory_class="TATA_box"
     regulatory      3772..3778
                     /regulatory_class="TATA_box"
     CDS             3866..5515
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(5559..5680)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(6099..6687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6861..7718)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7719..7823)
                     /label=AmpR promoter
     rep_origin      7850..8305
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    8436..8484
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    8498..8589
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"