pGJ103 vector (V005976)

Basic Vector Information

Vector Name:
pGJ103
Antibiotic Resistance:
Chloramphenicol
Length:
3284 bp
Type:
Expression shuttle vector
Replication origin:
ori
Source/Author:
Ming-Ming Y, Wei-Wei Z, Xi-Feng Z, Pei-Lin C.

pGJ103 vector Map

pGJ1033284 bp6001200180024003000Chloramphenicol acetyltransferaseoriKS primerSK primer

pGJ103 vector Sequence

LOCUS       V005976                 3284 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005976
VERSION     V005976
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ming-Ming Y, Wei-Wei Z, Xi-Feng Z, Pei-Lin C.
  TITLE     Construction and characterization of a novel maltose inducible
            expression vector in Bacillus subtilis
  JOURNAL   Biotechnol. Lett. 28 (21), 1713-1718 (2006)
   PUBMED   17001500
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Yang MM, Zhang WW.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2015) College of Animal Sciences and Technology,
            Northwest A
REFERENCE   3  (bases 1 to 3284)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3284)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Lett."; date: "2006"; volume: "28"; issue: "21"; pages: "1713-1718"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-DEC-2015) College of Animal Sciences and Technology, Northwest
            A"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1193..1837
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00486"
     rep_origin      complement(2003..2591)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     2643..2659
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(2693..2709)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"

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