pGFPTA vector (V006030)

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LOCUS       40924_21618        3249 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGFPTA DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ito Y, Suzuki M, Husimi Y.
  TITLE     A T-extended vector using a green fluorescent protein as an 
            indicator
  JOURNAL   Gene 245 (1), 59-63 (2000)
  PUBMED    10713445
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Ito Y, Suzuki M, Husimi Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2000) Yuzuru Husimi, Saitama University, 
            Deptartment of Functional Materials Science; 255 Shimo-ookubo, 
            Urawa, Saitama 338-8750, Japan 
            (E-mail:ito@evolve.fms.saitama-u.ac.jp, Tel:+81-48-858-3531, 
            Fax:+81-48-858-3531)
REFERENCE   3  (bases 1 to 3249)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3249)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "2000"; volume: "245"; issue: "1"; pages: "59-63"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-FEB-2000) Yuzuru Husimi, Saitama University, Deptartment of 
            Functional Materials Science"; volume: " 255 Shimo-ookubo, Urawa, 
            Saitama 338-8750, Japan (E-mail:ito@evolve.fms.saitama-u.ac.jp, 
            Tel:+81-48-858-3531, Fax"; pages: "+81-48-858-3531"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    52..73
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        88..118
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    126..142
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     150..166
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             234..947
                     /codon_start=1
                     /label=GFPuv
                     /note="GFP variant optimized for excitation by UV light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALLKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    952..957
                     /label=EcoRI/PvuII restriction site
                     /note="EcoRI/PvuII restriction site"
     promoter        1058..1162
                     /label=AmpR promoter
     CDS             1163..2020
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2194..2782
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2968..3108)
                     /label=bom
                     /note="basis of mobility region from pBR322"