Basic Vector Information
- Vector Name:
- pGFPBSDZ-SW2
- Antibiotic Resistance:
- Blasticidin
- Length:
- 10764 bp
- Type:
- Transformation vector
- Replication origin:
- ori
- Source/Author:
- Ding H, Gong S, Tian Y, Yang Z, Brunham R, Zhong G.
pGFPBSDZ-SW2 vector Vector Map
pGFPBSDZ-SW2 vector Sequence
LOCUS V006034 10764 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V006034
VERSION V006034
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 10764)
AUTHORS Ding H, Gong S, Tian Y, Yang Z, Brunham R, Zhong G.
TITLE Transformation of Sexually Transmitted Infection-Causing Serovars of
Chlamydia trachomatis Using Blasticidin for Selection
JOURNAL PLoS ONE 8 (11), E80534 (2013)
PUBMED 24303023
REFERENCE 2 (bases 1 to 10764)
AUTHORS Zhong G, Gong S, Ding H.
TITLE Direct Submission
JOURNAL Submitted (01-OCT-2013) Microbiology and Immunology, University of
Texas Health Science Center San Antonio, 7703 Floyd Curl Drive, San
Antonio, TX 78229, USA
REFERENCE 3 (bases 1 to 10764)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 10764)
AUTHORS .
TITLE Direct Submission
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
date: "2013"; volume: "8"; issue: "11"; pages: "E80534"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(01-OCT-2013) Microbiology and Immunology, University of Texas
Health Science Center San Antonio, 7703 Floyd Curl Drive, San
Antonio, TX 78229, USA"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..10764
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 1..88
/note="putative; contains 4 copies of a 22bp repeat"
CDS 165..680
/codon_start=1
/locus_tag="PSW2_1"
/product="putative virulence integrase pGP7-D"
/label="PSW2_1"
/protein_id="AHD24922.1"
/translation="MGSMAFHKSRLFLTFGDASEIWLSTLSYLTRKNYASGINFLVSLE
ILDLSETLIKAISLDHSESLFKIKSLDVFNGKVVSEASKQARAACYISFTKFLYRLTKG
YIKPAIPLKDFGNTTFFKIRDKIKTESISKQEWTVFFEALRIVNYRDYLIGKLIVQGIP
GYRARIHR"
gene 165..680
/locus_tag="PSW2_1"
/label="PSW2_1"
promoter complement(694..712)
/label="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
promoter 1058..1162
/label="AmpR promoter"
CDS 1163..1534
/label="BleoR"
/note="antibiotic-binding protein"
rep_origin 1708..2296
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 2554..2572
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 3100..3804
/label="GFP (S65T)"
/note="S65T variant of Aequorea victoria green fluorescent
protein (Heim et al., 1995)"
CDS 3805..4200
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label="BSD"
/note="confers resistance to blasticidin"
/translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
AIVKDSDGQPTAVGIRELLPSGYVWEG"
CDS complement(4351..5343)
/codon_start=1
/locus_tag="PSW2_2"
/product="virulence integrase pGP8-D"
/label="PSW2_2"
/protein_id="AHD24925.1"
/translation="MGKGILSLQQEMSLEYSEKSYQEVLKIRQESYWKRMKSFSLFEVI
MHWTASLNKHTCRSYRGSFLSLEKIGLLSLDMNLQEFSLLNHNLILDAIKKVSSAKTSW
TEGTKQVRAASYISLTRFLNRMTQGIVAIAQPSKQENSRTFFKTREIVKTDAMNSLQTA
SFLKELKKINARDWLIAQTMLQGGKRSSEVLSLEISQICFQQATISFSQLKNRQTEKRI
IITYPQKFMHFLQEYIGQRRGFVFVTRSGKMVGLRQIARTFSQAGLQAAIPFKITPHVL
RATAVTEYKRLGCSDSDIMKVTGHATAKMIFAYDKSSREDNASKKLALI"
gene complement(4351..5343)
/locus_tag="PSW2_2"
/label="PSW2_2"
CDS 5486..6838
/note="Probable plasmid replicative DNA helicase from
Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC
VR-902B). Accession#: B0BCM3"
/label="Probable plasmid replicative DNA helicase"
CDS 6835..7899
/codon_start=1
/locus_tag="PSW2_4"
/product="putative virulence protein pGP2-D"
/label="PSW2_4"
/protein_id="AHD24927.1"
/translation="MVNYSNCHFIKSPIHLENQKFGRRPGQSIKISPKLAQNGMVEVIG
LDFLSSHYHALAAIQRLLTATNYKGNTKGVVLSRESNSFQFEGWIPRIRFTKTEFLEAY
GVKRYKTSRNKYEFSGKEAETALEALYHLGHQPFLIVATRTRWTNGTQIVDRYQTLSPI
IRIYEGWEGLTDEENIDIDLTPFNSPPTRKHKGFVVEPCPILVDQIESYFVIKPANVYQ
EIKMRFPNASKYAYTFIDWVITAAAKKRRKLTKDNSWPENLLLNVNVKSLAYILRMNRY
ICTRNWKKIELAIDKCIEIAIKLGWLSRRKRIEFLDSSKLSKKEILYLNKERFEEITKK
SKEQMEQLEQESIN"
gene 6835..7899
/locus_tag="PSW2_4"
/label="PSW2_4"
CDS 7961..8755
/codon_start=1
/locus_tag="PSW2_5"
/product="putative virulence protein pGP3-D"
/label="PSW2_5"
/protein_id="AHD24928.1"
/translation="MGNSGFYLYNTENCVFADNIKVGQMTEPLKDQQIILGTTSTPVAA
KMTASDGISLTVSNNSSTNASITIGLDAEKAYQLILEKLGDQILDGIADTIVDSTVQDI
LDKIKTDPSLGLLKAFNNFPITNKIQCNGLFTPSNIETLLGGTEIGKFTVTPKSSGSMF
LVSADIIASRMEGGVVLALVREGDSKPCAISYGYSSGIPNLCSLRTSITNTGLTPTTYS
LRVGGLESGVVWVNALSNGNDILGITNTSNVSFLEVIPQTNA"
gene 7961..8755
/locus_tag="PSW2_5"
/label="PSW2_5"
CDS 8825..9133
/codon_start=1
/locus_tag="PSW2_6"
/product="putative virulence protein pGP4-D"
/label="PSW2_6"
/protein_id="AHD24929.1"
/translation="MQNKRKVRDDFIKIVKDVKKDFPELDLKIRVNKEKVTFLNSPLEL
YHKSVSLILGLLQQIENSLGLFPDSPVLEKLEDNSLKLKKALIMLILSRKDMFSKAE"
gene 8825..9133
/locus_tag="PSW2_6"
/label="PSW2_6"
CDS 9161..9955
/codon_start=1
/locus_tag="PSW2_7"
/product="putative ParA family partitioning protein pGP5-D"
/label="PSW2_7"
/protein_id="AHD24930.1"
/translation="MHTLVFCSFKGGTGKTTLSLNVGCNLAQFLGKKVLLADLDPQSNL
SSGLGASVRSDQKGLHDIVYTSNDLKSIICETKKDSVDLIPASFSSEQFRELDIHRGPS
NNLKLFLNEYCAPFYDICIIDTPPSLGGLTKEAFVAGDKLIACLTPEPFSILGLQKIRE
FLSSVGKPEEEHILGIALSFWDDRNSTNQMYIDIIESIYKNKLFSTKIRRDISLSRSLL
KEDSVANVYPNSRAAEDILKLTHEIANILHIEYERDYSQRTT"
gene 9161..9955
/locus_tag="PSW2_7"
/label="PSW2_7"
CDS 9952..10695
/codon_start=1
/locus_tag="PSW2_8"
/product="putative virulence protein pGP6-D"
/label="PSW2_8"
/protein_id="AHD24931.1"
/translation="MNKLKKEADVFFKKNQTAASLDFKKTLPSIELFSATLNSEESQSL
DRLFLSESQNYSDEEFYQEDILAVKLLTGQIKSIQKQHVLLLGEKIYNARKILSKDHFS
STTFSSWIELVFRTKSSAYNALAYYELFINLPNQTLQKEFQSIPYKSAYILAARKGDLK
TKVDVIGKVCGMSNSSAIRVLDQFLPSSRNKDVRETIDKSDSEKNRQLSDFLIEILRIM
CSGVSLSSYNENLLQQLFELFKQKS"
gene 9952..10695
/locus_tag="PSW2_8"
/label="PSW2_8"
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