pGFPamy vector (V006036)

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LOCUS       V006036                 7390 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006036
VERSION     V006036
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Bisicchia P, Botella E, Devine KM.
  TITLE     Suite of novel vectors for ectopic insertion of GFP, CFP and IYFP
            transcriptional fusions in single copy at the amyE and bglS loci in
            Bacillus subtilis
  JOURNAL   Plasmid 64 (3), 143-149 (2010)
   PUBMED   20600285
REFERENCE   2  (bases 1 to 7390)
  AUTHORS   Bisicchia P.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2010) Trinity College Dublin, 13 Meades Terrace,
            Dublin 2, Ireland
REFERENCE   3  (bases 1 to 7390)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7390)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
            date: "2010"; volume: "64"; issue: "3"; pages: "143-149"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-MAY-2010) Trinity College Dublin, 13 Meades Terrace, Dublin 2,
            Ireland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     115..651
                     /label="amyE front"
                     /note="amyE front"
     terminator      complement(673..766)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(789..1502)
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     misc_feature    complement(1503..1538)
                     /label="LIC site"
                     /note="LIC site"
     CDS             1691..2338
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_recomb     2712..3748
                     /label="amyE back"
                     /note="amyE back"
     CDS             4942..5130
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    5235..5375
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5561..6149)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6323..7180)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7181..7285)
                     /label="AmpR promoter"