pGEX-PUC-3T vector (V006049)

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LOCUS       cloning_vector;        3417 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGEX-PUC-3T DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    cloning vector; GST-fusion protein; GST-stuffer fusion protein
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Fukunaga R, Hunter T.
  TITLE     MNK1, a new MAP kinase-activated protein kinase, isolated by a novel
            expression screening method for identifying protein kinase 
            substrates
  JOURNAL   EMBO J. 16 (8), 1921-1933 (1997)
  PUBMED    9155018
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Fukunaga R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-1998) Rikiro Fukunaga, Osaka University Medical 
            School, Department of Genetics B-3; 2-2 Yamadaoka, Suita, Osaka 
            565-0871, Japan (E-mail:fukunaga@genetic.med.osaka-u.ac.jp, 
            Tel:81-6-879-3318, Fax:81-6-879-3319)
REFERENCE   3  (bases 1 to 3417)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3417)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "1997"; volume: "16"; issue: "8"; pages: "1921-1933"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-MAY-1998) Rikiro Fukunaga, Osaka University Medical School, 
            Department of Genetics B-3"; volume: " 2-2 Yamadaoka, Suita, Osaka 
            565-0871, Japan (E-mail:fukunaga@genetic.med.osaka-u.ac.jp, 
            Tel:81-6-879-3318, Fax"; pages: "81-6-879-3319"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..140
                     /label=AmpR promoter
     CDS             141..998
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1172..1760
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2171..2199
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    2207..2223
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2246..2899
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             2906..2923
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     misc_feature    2966..3385
                     /note="Stuffer:that the stuffer DNA is derived from the
                     420bp PvuII fragment(1802-2221) of the human G-CSF receptor
                     cDNA (Acc# No.M38025)"