pGEX-MbPylRS_TetR vector (V006051)

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LOCUS       V006051                 5923 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006051
VERSION     V006051
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Zhang Y, Ptacin JL, Fisher EC, Caffaro CE, San Jose K, Feldman AW,
            Turner CR, Romesberg FE.
  TITLE     A semi-synthetic organism that stores and retrieves increased
            genetic information
  JOURNAL   Nature (2017) In press
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Zhang Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2017) Department of Chemistry, The Scripps
            Research Institute, 10550 N Torrey Pines Rd, BCC526, San Diego, CA
            92037, USA
REFERENCE   3  (bases 1 to 5923)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5923)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature
            (2017) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-SEP-2017) Department of Chemistry, The Scripps Research
            Institute, 10550 N Torrey Pines Rd, BCC526, San Diego, CA 92037,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        106..134
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    142..158
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             181..1437
                     /gene="pylS"
                     /label="Pyrrolysine--tRNA ligase"
                     /note="Pyrrolysine--tRNA ligase from Methanosarcina
                     barkeri. Accession#: Q6WRH6"
     promoter        1741..1845
                     /label="AmpR promoter"
     CDS             1846..2703
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2877..3465
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             3704..4324
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     terminator      complement(4349..4435)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        4751..4828
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4829..5908
                     /label="lacI"
                     /note="lac repressor"