Basic Vector Information
- Vector Name:
- pGETS109
- Antibiotic Resistance:
- Ampicillin
- Length:
- 12485 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Tsuge K, Matsui K, Itaya M.
- Promoter:
- tet
pGETS109 vector Map
pGETS109 vector Sequence
LOCUS 40924_21414 12485 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pGETS109 DNA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12485)
AUTHORS Tsuge K, Matsui K, Itaya M.
TITLE One step assembly of multiple DNA fragments with a designed order
and orientation in Bacillus subtilis plasmid
JOURNAL Nucleic Acids Res. 31 (21), E133 (2003)
PUBMED 14576333
REFERENCE 2 (bases 1 to 12485)
AUTHORS Itaya M, Tsuge K, Matsui K.
TITLE Direct Submission
JOURNAL Submitted (13-AUG-2003) Mitsuhiro Itaya, Mitsubishi Kagaku Institute
of Life Sciences; 11 Minamiooya, Machida, Tokyo 194-8511, Japan
(E-mail:ita@libra.ls.m-kagaku.co.jp, Tel:81-42-724-6254,
Fax:81-42-724-6317)
REFERENCE 3 (bases 1 to 12485)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 12485)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
Acids Res."; date: "2003"; volume: "31"; issue: "21"; pages: "E133"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(13-AUG-2003) Mitsuhiro Itaya, Mitsubishi Kagaku Institute of Life
Sciences"; volume: " 11 Minamiooya, Machida, Tokyo 194-8511, Japan
(E-mail:ita@libra.ls.m-kagaku.co.jp, Tel:81-42-724-6254, Fax";
pages: "81-42-724-6317"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..12485
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 10..38
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
CDS 86..1273
/label=TcR
/note="tetracycline efflux protein"
regulatory 2175..2202
/gene="tetL"
/standard_name="bsr promoter"
/label=bsr promoter
/regulatory_class="promoter"
regulatory 2175..2180
/gene="tetL"
/standard_name="bsr promoter"
/label=bsr promoter
/regulatory_class="minus_35_signal"
regulatory 2197..2202
/gene="tetL"
/standard_name="bsr promoter"
/label=bsr promoter
/regulatory_class="minus_10_signal"
regulatory 2481..2485
/gene="tetL"
/regulatory_class="ribosome_binding_site"
CDS 2493..3866
/label=TcR
/note="tetracycline efflux protein"
terminator 3938..4024
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 4116..4143
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
gene 4411..7265
/gene="repA"
/label=repA
regulatory 4411..4439
/gene="repA"
/standard_name="spac-1"
/label=spac-1
/regulatory_class="promoter"
regulatory 4411..4416
/gene="repA"
/standard_name="spac-1"
/label=spac-1
/regulatory_class="minus_35_signal"
regulatory 4434..4439
/gene="repA"
/standard_name="spac-1"
/label=spac-1
/regulatory_class="minus_10_signal"
protein_bind 4446..4466
/gene="repA"
/standard_name="lacO"
/label=lacO binding site
/bound_moiety="LacI"
protein_bind 4448..4464
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
regulatory 6015..6044
/gene="repA"
/regulatory_class="promoter"
regulatory 6015..6019
/gene="repA"
/regulatory_class="minus_35_signal"
regulatory 6039..6044
/gene="repA"
/regulatory_class="minus_10_signal"
regulatory 6062..6066
/gene="repA"
/regulatory_class="ribosome_binding_site"
CDS 6075..7265
/codon_start=1
/gene="repA"
/product="RepA protein"
/label=repA
/protein_id="BAD00174.1"
/translation="MNNEPVKRGKKNRWELNLPIMTYVVADDWIDKLGHETFTLWLRFH
TWVDREDELRDYDRIPRSFENIYKKTLGISKSKFYRLIKPLWEYGLIDIIEYEESNRNS
TKPKNIIVYEYPLHEIERKYKPLEKLRDWDKDYNSVSKELGKTGGRPRKKDSEEEPEKK
PEEVTKKKRKYKLKRVIHNGFKNETVEGFKNETVEGFKNETVTVSKIKPNNYSNIFNNL
SNISTNVSNNLLIDDDEEIENEPTGRTINRSLLFSQEDIKQAYQFINRFSVIQLRENFS
FDKHFEERLVCYLWKAGISTFYTHEISKMIKKIADYEKSKKGRLNPIRDRALYMVNGLV
MNRASSQSEHATYKLNQYKKQKEQEKQQQEQQRSRVPFYNWLEEREEQTEGQLPTT"
RBS 7602..7610
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
regulatory 8885..8913
/gene="lacI"
/standard_name="penP promoter"
/label=penP promoter
/regulatory_class="promoter"
regulatory 8885..8890
/gene="lacI"
/standard_name="penP promoter"
/label=penP promoter
/regulatory_class="minus_35_signal"
regulatory 8908..8913
/gene="lacI"
/standard_name="penP promoter"
/label=penP promoter
/regulatory_class="minus_10_signal"
regulatory 9062..9069
/gene="lacI"
/standard_name="penP RBS"
/label=penP RBS
/regulatory_class="ribosome_binding_site"
CDS 9079..10158
/label=lacI
/note="lac repressor"
misc_feature 10332..10472
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(10658..11246)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(11420..12277)
/label=AmpR
/note="beta-lactamase"
promoter complement(12278..12382)
/label=AmpR promoter
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