pGEM-WIZ vector (V006063)

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LOCUS       40924_21369        3130 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGEM-WIZ, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Bao S, Cagan R.
  TITLE     Fast cloning inverted repeats for RNA interference
  JOURNAL   RNA 12 (11), 2020-2024 (2006)
  PUBMED    17005926
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Bao S, Cagan R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2006) Molecular Biology and Pharmacology, 
            Washington University, 660 South Euclid Ave, St. Louis, MO 63110, 
            USA
REFERENCE   3  (bases 1 to 3130)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3130)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA"; date:
            "2006"; volume: "12"; issue: "11"; pages: "2020-2024"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-AUG-2006) Molecular Biology and Pharmacology, Washington 
            University, 660 South Euclid Ave, St. Louis, MO 63110, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    10..243
                     /label=multiple cloning region
                     /note="multiple cloning region"
     regulatory      33..42
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          77..150
                     /number=2
                     /note="white gene"
     promoter        complement(255..273)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(291..307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(315..331)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(339..369)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(384..405)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(693..1281)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1455..2312)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2313..2417)
                     /label=AmpR promoter
     rep_origin      complement(2495..2950)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3091..3107
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3114..3130
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"