pGEM-URA3 vector (V006064)

Download GeneBank File(.gb)

LOCUS       V006064                 4350 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006064
VERSION     V006064
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Wilson RB, Davis D, Mitchell AP.
  TITLE     Rapid hypothesis testing with Candida albicans through gene
            disruption with short homology regions
  JOURNAL   J. Bacteriol. 181 (6), 1868-1874 (1999)
   PUBMED   10074081
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Wilson RB, Davis D, Mitchell AP.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-1999) Microbiology, Columbia University, 701 W.
            168th St., New York, NY 10032, USA
REFERENCE   3  (bases 1 to 4350)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4350)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "1999"; volume: "181"; issue: "6"; pages:
            "1868-1874"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-AUG-1999) Microbiology, Columbia University, 701 W. 168th St.,
            New York, NY 10032, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(75..93)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(111..127)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(135..151)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(159..189)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(204..225)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(513..1101)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1275..2132)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2133..2237)
                     /label="AmpR promoter"
     rep_origin      complement(2318..2773)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2914..2930
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2937..2955
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2986..2995
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3409..4218
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"