pGCD21 vector (V006074)

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LOCUS       40924_21245        6680 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGCD21, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Dantas GC, Martins PMM., Ferreira H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2016) Bioquimica e Microbiologia, UNESP, Av. 24A, 
            1515, Rio Claro, SP 13506900, Brazil
REFERENCE   2  (bases 1 to 6680)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6680)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (04-FEB-2016) Bioquimica e Microbiologia, UNESP, Av. 24A, 1515, Rio 
            Claro, SP 13506900, Brazil"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(98..973)
                     /label=araC
                     /note="L-arabinose regulatory protein"
     promoter        1000..1284
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     regulatory      1305..1310
                     /regulatory_class="ribosome_binding_site"
     CDS             1331..2044
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     misc_feature    complement(2081..2895)
                     /label=Xac_amy
                     /note="Xac_amy"
     primer_bind     complement(2908..2924)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3065..3493
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             3837..4628
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             4649..5506
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5680..6268
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6556..6577
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6592..6622
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6630..6646
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6654..6670
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"