pGBT-R15 vector (V006081)

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LOCUS       40924_21205        5469 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGBT-R15, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Clark KJ, Balciunas D, Pagoda H-M., Ding Y, Westcot SE, Bedell VM, 
            Greenwood TM, Urban MD, Skuster KJ, Petzold AM, Ni J, Nielson A, 
            Sivasubbu S, Xu X, Hammerschmidt M, Ekker SC.
  TITLE     In vivo protein trapping produces a functional expression codex of 
            the vertebrate proteome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Clark KJ, Balciunas D, Pagoda H-M., Ding Y, Westcot SE, Bedell VM, 
            Greenwood TM, Urban MD, Skuster KJ, Petzold AM, Ni J, Nielson A, 
            Sivasubbu S, Xu X, Hammerschmidt M, Ekker SC.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-OCT-2010) Biochemistry and Molecular Biology, Mayo 
            Clinic, 221 4th Ave. SW, Rochester, MN 55902, USA
REFERENCE   3  (bases 1 to 5469)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5469)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-OCT-2010) Biochemistry and Molecular Biology, Mayo Clinic, 221 
            4th Ave. SW, Rochester, MN 55902, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  complement(1..192)
                     /mobile_element_type="transposon:Tol2"
                     /label=ol2
                     /note="mini Tol2 ITR-R"
     protein_bind    221..254
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          298..395
                     /note="carp beta-actin intron 1 splice acceptor"
     CDS             428..1099
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     regulatory      1112..2116
                     /label=Danio rerio beta-actin 3'UTR and poly(A) signal
                     /note="Danio rerio beta-actin 3'UTR and poly(A) signal"
                     /regulatory_class="polyA_signal_sequence"
     rep_origin      1588..2044
                     /label=F1 Ori
                     /note="F1 Ori"
     misc_recomb     2213..2246
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(2213..2246)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     mobile_element  2269..2530
                     /mobile_element_type="transposon:Tol2"
                     /label=ol2
                     /note="mini Tol2 ITR-L"
     promoter        complement(2543..2561)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2568..2584)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2725..3180
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3258..3362
                     /label=AmpR promoter
     CDS             3363..4220
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4394..4982
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5270..5291
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5306..5336
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5344..5360
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5368..5384
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5402..5420
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"