pGBKCg vector (V006087)

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LOCUS       40924_21170        9011 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast two-hybrid vector pGBKCg, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9011)
  AUTHORS   Stellberger T, Uetz P.
  TITLE     Yeast two-hybrid vectors with C-terminal fusion of Gal4 activation 
            and DNA-binding domains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9011)
  AUTHORS   Stellberger T, Uetz P.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2009) Institut fuer Toxikologie und Genetik, 
            Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1, 
            Eggenstein-Leopoldshafen, Baden-Wuerttemberg 76344, Germany
REFERENCE   3  (bases 1 to 9011)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9011)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-FEB-2009) Institut fuer Toxikologie und Genetik, 
            Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1, 
            Eggenstein-Leopoldshafen, Baden-Wuerttemberg 76344, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..734
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     protein_bind    769..888
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        918..948
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1002..1658
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2003..2305
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2349..2473)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2487..2927
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     promoter        2938..2956
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2976..3005
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     terminator      complement(3093..3140)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     regulatory      3143..3310
                     /label=yADH1_terminator
                     /note="yADH1_terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(3330..3346)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3354..3370)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3378..3408)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3423..3444)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3732..4320)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    complement(4649..4696)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(4932..5723)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5724..5828)
                     /label=AmpR promoter
     rep_origin      complement(5855..7197)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        7456..7737
                     /label=TRP1 promoter
     CDS             7738..8409
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
     rep_origin      complement(8515..8970)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"