pGATA vector (V006088)

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LOCUS       40924_21135        5329 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGATA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Trudel P, Provost S, Massie B, Chartrand P, Wall L.
  TITLE     pGATA: a positive selection vector based on the toxicity of the 
            transcription factor GATA-1 to bacteria
  JOURNAL   BioTechniques 20 (4), 684-693 (1996)
  PUBMED    8800690
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   Trudel P, Chartrand P, Wall L, Ching RW.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2006) Biochemistry, University of Toronto, 555 
            University Avenue, Toronto, ON M5G 1X8, Canada
REFERENCE   3  (bases 1 to 5329)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5329)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "1996"; volume: "20"; issue: "4"; pages: 
            "684-693"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-SEP-2006) Biochemistry, University of Toronto, 555 University 
            Avenue, Toronto, ON M5G 1X8, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..211
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    219..235
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        691..709
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    722..829
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(838..856)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1095..1112
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     misc_feature    1110..1319
                     /note="fragment of murine GATA-1; contains the second zinc 
                     finger and basic regions of the protein"
     promoter        1632..1736
                     /label=AmpR promoter
     CDS             1737..2594
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2768..3356
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3600..3677
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3678..4757
                     /label=lacI
                     /note="lac repressor"
     protein_bind    4773..4794
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4809..4839
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4847..4863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4883..5056
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"