Basic Vector Information
- Vector Name:
- pGapneoR3
- Antibiotic Resistance:
- Kanamycin
- Length:
- 9101 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Zhang J.
pGapneoR3 vector Map
pGapneoR3 vector Sequence
LOCUS 40924_21100 9101 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pGapneoR3, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9101) AUTHORS Zhang J. TITLE Direct Submission JOURNAL Submitted (17-AUG-2016) Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning 125100, China REFERENCE 2 (bases 1 to 9101) TITLE Direct Submission REFERENCE 3 (bases 1 to 9101) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (17-AUG-2016) Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning 125100, China" COMMENT SGRef: number: 2; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..9101 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 6..23 /label=6xHis /note="6xHis affinity tag" terminator 58..310 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" misc_feature 332..356 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 1656..2282 /label=pVS1 StaA /note="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS 2714..3784 /label=pVS1 RepA /note="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" rep_origin 3853..4047 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" misc_feature 4391..4531 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(4717..5305) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5395..6186) /label=KanR /note="aminoglycoside phosphotransferase" misc_feature 6611..6635 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" CDS complement(6745..7533) /label=NeoR/KanR /note="aminoglycoside phosphotransferase" regulatory complement(7589..9027) /regulatory_class="promoter"
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