pGapneoR3 vector (V006089)

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LOCUS       40924_21100        9101 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGapneoR3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   Zhang J.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2016) Research Institute of Pomology, Chinese 
            Academy of Agricultural Sciences, Xingcheng, Liaoning 125100, China
REFERENCE   2  (bases 1 to 9101)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9101)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (17-AUG-2016) Research Institute of Pomology, Chinese Academy of 
            Agricultural Sciences, Xingcheng, Liaoning 125100, China"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6..23
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      58..310
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    332..356
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1656..2282
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2714..3784
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      3853..4047
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4391..4531
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4717..5305)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5395..6186)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    6611..6635
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(6745..7533)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(7589..9027)
                     /regulatory_class="promoter"