pGAPDH.Rep68 vector (V006091)

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LOCUS       V006091                 5439 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006091
VERSION     V006091
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   van Nierop GP, de Vries AA, Holkers M, Vrijsen KR, Goncalves MA.
  TITLE     Stimulation of homology-directed gene targeting at an endogenous
            human locus by a nicking endonuclease
  JOURNAL   Nucleic Acids Res. 37 (17), 5725-5736 (2009)
   PUBMED   19651880
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   van Nierop GP, de Vries AA, Holkers M, Vrijsen KR, Goncalves MA.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2009) Molecular Cell Biology, Leiden University
            Medical Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The
            Netherlands
REFERENCE   3  (bases 1 to 5439)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5439)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "2009"; volume: "37"; issue: "17"; pages:
            "5725-5736"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-JUL-2009) Molecular Cell Biology, Leiden University Medical
            Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The
            Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             8..1615
                     /gene="Rep68"
                     /label="Protein Rep68"
                     /note="Protein Rep68 from Adeno-associated virus 2 (isolate
                     Srivastava/1982). Accession#: P03132"
     polyA_signal    1688..1736
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(2223..2239)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2713..2817
                     /label="AmpR promoter"
     CDS             2818..3675
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3849..4437
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4725..4746
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4761..4791
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4799..4815
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4823..4839
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"