Basic Vector Information
- Vector Name:
- pGal4-p65f1-539
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6147 bp
- Type:
- Mammalian expression vector
- Replication origin:
- ori
- Source/Author:
- De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.
- Promoter:
- RSV
pGal4-p65f1-539 vector Map
pGal4-p65f1-539 vector Sequence
LOCUS V006110 6147 bp DNA circular SYN 18-DEC-2018 DEFINITION Exported. ACCESSION V006110 VERSION V006110 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 6147) AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished REFERENCE 2 (bases 1 to 6147) AUTHORS De Schamphelaire W. TITLE Direct Submission JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM REFERENCE 3 (bases 1 to 6147) TITLE Direct Submission REFERENCE 4 (bases 1 to 6147) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM" SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..6147 /mol_type="other DNA" /organism="synthetic DNA construct" polyA_signal complement(766..900) /label="SV40 poly(A) signal" /note="SV40 polyadenylation signal" CDS complement(924..2540) /gene="RELA" /label="Transcription factor p65" /note="Transcription factor p65 from Homo sapiens. Accession#: Q04206" misc_feature complement(2541..2576) /label="link" /note="link" CDS complement(2577..3017) /label="GAL4 DNA binding domain" /note="DNA binding domain of the GAL4 transcriptional activator" promoter complement(3159..3420) /label="RSV promoter" /note="Rous sarcoma virus enhancer/promoter" promoter 3745..4074 /label="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin complement(4320..4908) /direction=LEFT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5082..5939) /label="AmpR" /note="beta-lactamase" promoter complement(5940..6044) /label="AmpR promoter"
This page is informational only.