pGal4-p65f1-539 vector (V006110)

Basic Vector Information

Vector Name:
pGal4-p65f1-539
Antibiotic Resistance:
Ampicillin
Length:
6147 bp
Type:
Mammalian expression vector
Replication origin:
ori
Source/Author:
De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.
Promoter:
RSV

pGal4-p65f1-539 vector Map

pGal4-p65f1-5396147 bp30060090012001500180021002400270030003300360039004200450048005100540057006000SV40 poly(A) signalTranscription factor p65linkGAL4 DNA binding domainRSV promoterSV40 promoteroriAmpRAmpR promoter

pGal4-p65f1-539 vector Sequence

LOCUS       V006110                 6147 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006110
VERSION     V006110
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6147)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6147)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(766..900)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(924..2540)
                     /gene="RELA"
                     /label="Transcription factor p65"
                     /note="Transcription factor p65 from Homo sapiens.
                     Accession#: Q04206"
     misc_feature    complement(2541..2576)
                     /label="link"
                     /note="link"
     CDS             complement(2577..3017)
                     /label="GAL4 DNA binding domain"
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     promoter        complement(3159..3420)
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     promoter        3745..4074
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(4320..4908)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5082..5939)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5940..6044)
                     /label="AmpR promoter"

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